Altered E2 glycoprotein of Sindbis virus and its use in complementation studies
نویسندگان
چکیده
منابع مشابه
Carbohydrate structure of Sindbis virus glycoprotein E2 from virus grown in hamster and chicken cells.
Sindbis virus was used as a probe to examine glycosylation processes in two different species of cultured cells. Parallel studies were carried out analyzing the carbohydrate added to Sindbis glycoprotein E2 when the virus was grown in chicken embryo cells and BHK cells. The Pronase glycopeptides of Sindbis glycoprotein E2 were purified by a combination of ion-exchange and gel filtration chromat...
متن کاملMutations in the endodomain of Sindbis virus glycoprotein E2 define sequences critical for virus assembly.
Envelopment of Sindbis virus at the plasma membrane is a multistep process in which an initial step is the association of the E2 protein via a cytoplasmic endodomain with the preassembled nucleocapsid. Sindbis virus is vectored in nature by blood-sucking insects and grows efficiently in a number of avian and mammalian vertebrate hosts. The assembly of Sindbis virus, therefore, must occur in two...
متن کاملMolecular links between the E2 envelope glycoprotein and nucleocapsid core in Sindbis virus.
A three-dimensional reconstruction of Sindbis virus at 7.0 Å resolution presented here provides a detailed view of the virion structure and includes structural evidence for key interactions that occur between the capsid protein (CP) and transmembrane (TM) glycoproteins E1 and E2. Based on crystal structures of component proteins and homology modeling, we constructed a nearly complete, pseudo-at...
متن کاملMutational analysis of a virulence locus in the E2 glycoprotein gene of Sindbis virus.
The substitution of arginine for serine at position 114 of glycoprotein E2 in several biological and recombinant Sindbis virus mutants was shown previously to attenuate the virus for neonatal mice and also to accelerate virus penetration into BHK cells. To further examine the genetically linked effects on both virus penetration into cultured cells and pathogenesis in vivo, mutants containing ea...
متن کاملXgtll Expression Library To Localize a Neutralizing Antibody-Binding Site in Glycoprotein E2 of Sindbis Virus
The Sindbis virus envelope contains two species of integral membrane glycoproteins, El and E2. These proteins form heterodimers, and three dimeric units assemble to form spikes incorporated into the viral surface which play an important role in the specific attachment of Sindbis virus to host cells. To map the neutralization epitopes on the surface of the virus, we constructed a Agtll expressio...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1978
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.26.1.126-135.1978